CelLytic™ PN Isolation/Extraction Kit
SIGMA/CELLYTPN1 - For plant leaves
Synonym: Cell lysis kit
Product Type: Chemical
Figure 1. Detection of RNA polymerase II in Tomato Nuclear versus Cytoplasmic extracts, prepared with CelLytic-PN Kit.
The Extracts were run on SDS-PAGE and blot-hybridized to anti-RNA Polymerase II antibody.
The Extracts were run on SDS-PAGE and blot-hybridized to anti-RNA Polymerase II antibody.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | ChIP: suitable |
| usage | kit sufficient for 30 extractions (20 g of fresh or frozen leaves) |
| Application: | The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis. |
| Features and Benefits: | Efficient Nuclei Isolation and Protein Extraction: CelLytic™ PN Isolation/Extraction Kit provides a fast and efficient method for isolating nuclei and extracting functional nuclear proteins from plant leaves, saving time and effort for academic researchers. Versatility: The isolated nuclei can be used as a source for chromatin, genomic DNA, RNA, etc., making CelLytic™ PN Isolation/Extraction Kit a versatile solution for plant molecular biology research. Suitable for Detection of DNA-Protein Interactions: The nuclear protein extract obtained using this kit is ideal for detecting DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis, western blot assay, and similar techniques, which is crucial for researchers studying transcription factors and chromatin remodeling. Plant Model Specific Protocol: The CelLytic™ PN Isolation/Extraction Kit offers a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis, making it an easy-to-follow solution for researchers working with these plant models. Rapid Extraction: The CelLytic™ PN Isolation/Extraction Kit provides academic researchers with a rapid extraction method, allowing them to quickly extract functional nuclear proteins from plant leaves for their research. |
| General description: | CelLytic™-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones. |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves. |
| Symbol |    GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H410 |
| Precautionary statements | P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn,N |
| Risk Statements | 22-36-51/53 |
| Safety Statements | 26-61 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 41116134 |