Catalase Assay Kit

SIGMA/CAT100 - sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

Synonym: Catalase Activity Detection Kit

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-CAT100-1KT	1 kit	$996.00 1/EA		Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

detection method	colorimetric
Quality Level	200
shipped in	wet ice
storage temp.	2-8°C
usage	sufficient for ≥100 tests enzymatic

Application:	Suitable for studying catalase activity in various tissues and subcellular organelles.
Application:	Sutitable for Colorimetric and UV Assays
Biochem/physiol Actions:	Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H₂O₂ from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.
Biochem/physiol Actions:	This assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nm
Features and Benefits:	• Useful for determining catalase activity - may be used in various tissues and cells • Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity
General description:	The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.
Other Notes:	One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.
Preparation Note:	Use ultrapure water in preparation of all solutions.

Symbol	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261 - P284 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12161503
Components	Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), ≥250 units/mg solid (using pyrogallol) 5 mg; Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H₂O

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