Calcium Phosphate Transfection Kit
SIGMA/CAPHOS - Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
Synonym: Gene delivery
EC Number: 233-140-8
Product Type: Chemical
Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.
| form | solution |
| grade | Molecular Biology |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | transfection: suitable |
| usage | kit sufficient for 160 transfections (6 cm dishes) |
| kit sufficient for 400 transfections (3.5 cm dishes) | |
| kit sufficient for 80 transfections (10 cm dishes) |
| Application: | Calcium Phosphate Transfection Kit has been used: • to enable transfection • to transfect Hek293T cells • to transfect H29D cells |
| Application: | Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method: BAEC Bowels melanoma cells CHO K1 COS-7 Fibroblasts (human embryonic, neo derm) HEK293 Huh 7 IMR-90 LLC (Lewis Lung Carcinoma) NIH3T3 PC-12 PCI-13 SH-Sy5Y SK-Hep-1 T47D |
| Biochem/physiol Actions: | The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis. |
| Features and Benefits: | • Suitable for transient and stable transfection • Reproducible for a wide range of cell types • Widely referenced • Inexpensive |
| General description: | Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types. |
| Other Notes: | The Calcium Phosphate Transfection Kit contains: 5 ml 2.5M CaCl2 (C2052) 25 ml 2x HEPES Buffered Saline (H1012) 25 ml molecular biology grade water (W4502) |
| Other Notes: | The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 22-24 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |