Cholera Toxin B subunit
SIGMA/C9972 - biotin conjugate, lyophilized powder
Synonym: CTB
MDL Number: MFCD02356522
Product Type: Chemical
ELISA ELISA plate coated with Monosialoganglioside GM1 from Bovine brain (Cat. No. G7641) incubated with serially diluted Cholera Toxin B subunit (Cat. No. C9972) and Anti-Cholera Toxin antibody produced in Rabbit (Cat. No. C3062) followed by Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545) to Determine Cholera Toxin B subunit binding to Monosialoganglioside GM1 by indirect ELISA
| composition | Protein, ~40% Lowry |
| conjugate | biotin conjugate |
| form | lyophilized powder |
| InChI | 1S/C16H13ClF3NO2/c1-2-23- |
| InChI key | YDXZSNHARVUYNM-UHFFFAOYSA |
| mol wt | ~12 kDa |
| Quality Level | 200  |
| SMILES string | CCOc1ccccc1C(=O)Nc2ccc(Cl |
| storage temp. | 2-8°C |
| Analysis Note: | Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared. |
| Analysis Note: | Biotin content ~1.0 mole/mole protein. |
| Application: | Cholera Toxin B subunit has been used: • in immunofluorescence • in the analysis of major histocompatibility complex (MHC) class II lipid raft partitioning • in live cell three-dimensional tracking of SH-SY5Y human neuroblastoma cells • to assess the toll-like receptors (TLR) and FcRγ (Fc receptor γ chain) – CARD9 (caspase recruitment domain family member 9) activation by cholera Toxin B (CTB) |
| Biochem/physiol Actions: | The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. |
| Biochem/physiol Actions: | The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
| General description: | Cholera Toxin B (CTB) is secreted by Vibrio cholerae. CTB functions as an oral subunit vaccine for cholera, which is associated with acute watery diarrhoea. It acts as a mucosal immunogen. Cholera toxin (CT) stimulates cell surface molecules, such as antigen presenting cells (APCs), murine and human dendritic cells (DCs). CT also has immunomodulatory properties. It induces the secretion of interleukin 1 (IL-1) from macrophages and enhances their APC function. |
| Packaging: | 0.5 mg in serum bottle |
| Physical form: | Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |