Cholera Toxin B subunit
SIGMA/C9903 - ≥95% (SDS-PAGE), lyophilized powder
Synonym: CTB
MDL Number: MFCD02356522
Product Type: Chemical
ELISA ELISA plate coated with Monosialoganglioside GM1 from Bovine brain (Cat. No. G7641) incubated with serially diluted Cholera Toxin B subunit (Cat. No. C9903) and Anti-Cholera Toxin antibody produced in Rabbit (Cat. No. C3062) followed by Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545) to Determine Cholera Toxin B subunit binding to Monosialoganglioside GM1 by indirect ELISA
| assay | ≥95% (SDS-PAGE) |
| composition | Protein, ~5% Lowry |
| form | lyophilized powder |
| impurities | ≤0.5% Cholera toxin A subunit (SDS-PAGE) |
| InChI | 1S/C16H13ClF3NO2/c1-2-23- |
| InChI key | YDXZSNHARVUYNM-UHFFFAOYSA |
| mol wt | ~12 kDa |
| Quality Level | 300  |
| SMILES string | CCOc1ccccc1C(=O)Nc2ccc(Cl |
| solubility | H2O: 10 mg/mL |
| storage temp. | 2-8°C |
| Analysis Note: | Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL. |
| Application: | Cholera Toxin B subunit has been used: • for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4. • in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells. |
| Biochem/physiol Actions: | The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. |
| Biochem/physiol Actions: | The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
| Features and Benefits: | This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm . |
| General description: | Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries. |
| Packaging: | Package size based on protein content |
| Physical form: | Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA. |
| Preparation Note: | When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H312 + H332 - H412 |
| Precautionary statements | P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |