Chitosanase from Streptomyces griseus
SIGMA/C9830 - lyophilized powder, >50 units/mg protein (Bradford)
Synonym: Chitosan N-
CAS Number: 51570-20-8
MDL Number: MFCD00211797
Product Type: Chemical
Chitosanase specificity: Chitosan is a polymer of β-(1-4)-N--acetyl-D-glucosamine units.
| foreign activity | chitinase <1.0 units/mg protein |
| form | lyophilized powder |
| purified by | chromatography |
| Quality Level | 200  |
| solubility | H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL) |
| specific activity | >50 units/mg protein (Bradford) |
| storage temp. | −20°C |
| Application: | Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan. |
| Application: | Chitosanase from Streptomyces griseus has been used in the digestion of lacrimal gland (LG) tissues. |
| Application: | The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel. |
| Biochem/physiol Actions: | Chitosanase catalyzes the endohydrolysis of N-acetylated chitosan specifically at the of β(1,4) linkages between N-acetyl- |
| General description: | Chitosanase is synthesized from Streptomyces griseus as an extracellular secreted enzyme and corresponds to a molecular weight of 34 kDa. It has a temperature and pH optimum of 60°C and 5.7, respectively. Chitosanase belongs to the glycosyl hydrolase family and comprises α-helical tertiary structure similar to lysozyme with substrate-binding cleft placed between two lobes. |
| Other Notes: | One unit will release 1 μmole of glucosamine from chitosan per min at pH 5.0 at 37 °C as measured in the fluorimetric assay of Osswald, et al. (1992). |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 10 units in serum bottle |
| Physical form: | Lyophilized powder containing potassium phosphate buffer salts |
| Preparation Note: | For long term solution storage, reconstitute vial with 50% glycerol solution. Dilute to working concentrations with a 0.1% BSA solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: >50 units/mg protein (Bradford) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.132 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |