Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse
SIGMA/C9672 - clone NCAM-0B11, ascites fluid
Synonym: Anti-CD56-CY; Anti-NCAM
MDL Number: MFCD00164707
Product Type: Chemical
Immunoblotting Rat brain cytosol S1 extract was separated on SDS-PAGE and probed with Monoclonal Anti-Neural Cell Adhesion Molecule Clone: NCAM-0B11 (Cat. No. C9672). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:100 2. Negative control: without 1st antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NCAM-0B11, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 140-180 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using cells and tissues |
| western blot: 1:100 using a freshly prepared extract from newborn and adult rat brain, or rat cerebral cortex extract | |
| UniProt accession no. | O15394  |
| P13591 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (3 papers) |
| Application: | Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse has been used in: • Immunofluorescence • Immunohistochemistry • Immunoblotting |
| Biochem/physiol Actions: | Neural cell adhesion molecule (N-CAM) are believed to be involved in cell-cell interactions and probably play an important role in embryogenesis and development. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-N-CAM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neural cell adhesion molecule (N-CAM), the best characterized CAM, exists in adult brain. It belongs to the family of sialoglycoproteins, which arise from alternative splicing of mRNA transcribed from a single gene. Immunocytochemical data indicate that in the adult, N-CAM is expressed mainly in cells of the nervous system. There are indications that N-CAM may also be expressed by non-neural cells in the adult, as it has been found in studies of various embryonic developmental stages. |
| General description: | Mouse monoclonal clone NCAM-0B11 anti-Neural Cell Adhesion Molecule antibody localizes the high molecular weight isoform of N-CAM (neural cell adhesion molecule) in human and several other mammalian species. The antibody shows a strong reaction to N-CAM A/N-CAM 180, however it also recognizes N-CAM B/N-CAM 140. In an immunoblot, the product reacts with polysialated N-CAM in embryonic material. Breakdown products of N-CAM may be stained; they appear as bands lower than 100 kD on the immunoblot. |
| Immunogen: | growth cone enriched plasma membrane fraction from E17 rat forebrain. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |