Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody, Mouse monoclonal
SIGMA/C9205 - clone G-A-5, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse; Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)−Cy3™ antibody produced in mouse; Anti-ALXDRD; Anti-GFAP
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Transplantation of autologous adipose stem cells lacks therapeutic efficacy in the experimental autoimmune encephalomyelitis model. By: Zhang, X., Bowles, A. C., et al. in PLoS One, 2014. PubMed ID: 24465465 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0085007), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded heat retrieval tissue section of rat brain stained with 1:400 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)-Cy3™ antibody produced in Mouse clone no. G-A-5 (Cat. No. C9205 ).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | G-A-5, monoclonal |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human, pig, rat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections |
| UniProt accession no. | P14136  |
| Application: | Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in • immunohistochemistry • immunoblotting • immunolabelling, • immunocytochemistry • immunofluorescence |
| Biochem/physiol Actions: | Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas. |
| Biochem/physiol Actions: | The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections. |
| Biochem/physiol Actions: | The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, Bergmann glia cells and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well. |
| Immunogen: | GFAP from pig spinal cord |
| Legal Information: | Cy is distributed under license from Amersham Biosciences Limited. |
| Legal Information: | Cy3 is a trademark of Cytiva |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |