Anti-Cdh1 antibody,Mouse monoclonal
SIGMA/C7855 - clone DCS-266, purified from hybridoma cell culture
Synonym: Anti-CDC20C; Anti-CDH1; Anti-FZR; Anti-FZR2; Anti-HCDH; Anti-HCDH1
MDL Number: MFCD03457549
Product Type: Chemical
Immunoblotting HeLa nuclear extract was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdh1 Clone: DCS-266 (Cat. No. C7855) and developed using Goat Anti-Mouse IgG (Fab)-Biotin (Cat. No. B0529) followed by ExtrAvidin-ALP (Cat. No. E2636) and NBT/BCIP (Cat. No. B5655) substrate. Lanes 1. 4 μg/mL antibody 2. Negative control: without antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DCS-266, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| microarray: suitable | |
| western blot: 2-4 μg/mL using a HeLa cell nuclear extract | |
| UniProt accession no. | Q9UM11  |
| Application: | Monoclonal Anti-Cdh1 antibody was used for immunoblotting of lysates in a study of Identification of Novel Human Cdt1-binding Proteins. It is suitable for western blotting with 2-4 μg/mL by using a HeLa cell nuclear extract. It is also suitable for immunoprecipitation and microarray. |
| Biochem/physiol Actions: | Monoclonal Anti-Cdh1 reacts specifically with human Cdh1. |
| Biochem/physiol Actions: | Monoclonal Anti-Cdh1 reacts specifically with human Cdh1. In eukaryotes, regulation of cell cycle progression depends on the expression of cyclins proteins. Degradation of mitotic cyclins follows ubiquitin-dependent pathways. The anaphase-promoting complex/cyclosome (APC/C) plays a vital role in progression. Initially at anaphase the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but later it require Cdh1 to maintain its active state till the next S phase. Another cyclin A/cdk2 mediated phosphorylation of Cdh1 may also prevents the activatory assembly of Cdh1 with APC, thus creating an environment permissive for accumulation of APC targets. It mediates Ca2+ dependent homophilic interactions, as the major adhesion receptor in adherens junctions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cdh1 homophilic major cell adhesion molecule in epithelial cells belongs to cadherin superfamily. |
| General description: | Monoclonal Anti-Cdh1 (mouse IgG1 isotype) is derived from the DCS-266 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human cadherin-1 (Cdh1). |
| Immunogen: | recombinant human Cdh1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |