Cytochrome c from equine heart
SIGMA/C7752 - ≥95% based on Mol. Wt. 12,384 basis
Synonym: Cytochrome c from horse heart
CAS Number: 9007-43-6
EC Number: 232-700-9
MDL Number: MFCD00130890
Product Type: Chemical
Cytochrome c is a heme protein containing a single polypeptide chain and a single heme group, which is covalently attached to Cys14 and Cys17.
The electron transport protein complex of the inner mitochondrial membrane
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7752-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7752-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C7752-50MG
| assay | ≥95% (SDS-PAGE) |
| ≥95% based on Mol. Wt. 12,384 basis | |
| biological source | horse heart |
| form | powder |
| mol wt | 12,384 |
| Quality Level | 300  |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P00004  |
| Application: | Cytochrome c is used as an inducer of mitochondria-dependent apoptosis in various research studies. The identification and monitoring of oxidation sites in cytochrome c may have implications for assessing oxidative stress-related conditions and diseases. It could potentially be used as a biomarker to evaluate the extent of oxidative damage and the antioxidant capacity of cells and tissues. Cytochrome c is suitable for use as a component of phosphate buffer for the detection and evaluation of complex IV activity using blue-native gel electrophoresis (BN-PAGE). It may also be used for the detection of extracellular superoxide anion (ECSA) in isolated kidney phagocytes. |
| Application: | The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell. |
| Biochem/physiol Actions: | Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. |
| Biochem/physiol Actions: | Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen |
| Other Notes: | View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enz. |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 50, 100, 500 mg in glass bottle |
| Preparation Note: | Prepared with acetic acid without using TCA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE); ≥95% based on Mol. Wt. 12,384 basis |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |