Chaperonin 10 from Escherichia coli
SIGMA/C7438 - ≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder
Synonym: GroES
MDL Number: MFCD00163256
Product Type: Chemical
SDS-PAGE Purity of Chaperonin 10 from Escherichia coli (Cat. No. C7438) was assessed on SDS-PAGE.
Folding activity of Gro ES (Chaperonin 10) Testing the folding activity of Gro ES system (Chaperonin 10 from Escherichia coli, Cat. No. C7438) and the refolding of Rhodanese by Gro ES. The Chaperonin protein Gro ES (Chaperonin 10) is a heat shock protein that assist the folding of nascent and heat destabilized proteins. In vitro Gro ES promote protein refolding and reactivation. Reconstitution of denatured enzymes such as the photosynthetic enzyme Rubisco1, mitochondrial Rhodanese2 and Glutamine synthase3 were reported. S1: Denaturated Rhodanese + Chaperonin 10 S2: Native Rhodanese + Chaperonin 10 S3: Denaturated Rhodanese S4: Native Rhodanese
| assay | ≥95.0% (SDS-PAGE) |
| biological source | Escherichia coli |
| form | lyophilized powder |
| Quality Level | 200  |
| recombinant | expressed in E. coli overproducing strain |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P61604  |
| Biochem/physiol Actions: | Chaperonin60 (GroEL) and chaperonin10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese. The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained. |
| Packaging: | Package size based on protein content. |
| Physical form: | Lyophilized powder containing Tris buffer salts, potassium chloride, dithiothreitol and trehalose as stabilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95.0% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |