Anti-Cdc27 antibody,Mouse monoclonal
SIGMA/C7104 - clone AF3.1, purified from hybridoma cell culture
MDL Number: MFCD01633250
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-Cdc27 Clone: AF3.1 (Cat. No. C7104). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. MDBK 3. COS7 4. NIH-3T3 5. P19
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AF3.1, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen 97 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, Xenopus, rat, bovine, canine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using a HeLa Cell nuclear extract | |
| UniProt accession no. | P30260  |
| Application: | Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications: • Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization • Immunohistochemistry (formalin-fixed, paraffin-embedded sections) • Immunoprecipitation. • Indirect ELISA • Microarray • Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract. • Anaphase-promoting complex/cyclosome APC/C Purification. |
| Biochem/physiol Actions: | CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer. |
| Biochem/physiol Actions: | The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH. |
| General description: | The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus. |
| Immunogen: | synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |