5-Chloro-2′-deoxyuridine
SIGMA/C6891 - thymidine analog
Synonym: CldU
CAS Number: 50-90-8
Empirical Formula (Hill Notation): C9H11ClN2O5
Molecular Weight: 262.65
MDL Number: MFCD00006531
Linear Formula: C9H11ClN2O5
Product Type: Chemical
ATR-IR Spectra for 5-Chloro-2′-deoxyuridine.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C6891-100MG
| assay | ≥98% (HPLC) |
| form | powder |
| InChI | 1S/C9H11ClN2O5/c10-4-2-12 |
| InChI key | NJCXGFKPQSFZIB-RRKCRQDMSA |
| Quality Level | 100  |
| SMILES string | OC[C@H]1O[C@H](C[C@@H]1O) |
| solubility | 1 M NH4OH: 20 mg/mL, clear, colorless to faintly yellow |
| storage temp. | −20°C |
| Application: | 5-Chloro-2′-deoxyuridine has been used to: • study cell cycle dynamics by immunohistochemical analysis and double S-phase labeling using animal models • analyze cell cycle length using an animal model • label HEK293T cells for molecular combing assay |
| Biochem/physiol Actions: | DNA labeled with 5-chloro-2′-deoxyuridine (CldU) serves as an effective tool to analyze and quantify DNA replication, repair, and recombination. CldU is a potent mutagen, clastogen, and toxicant. It is used as a thymidine analog and is found to alter the dNTP pools and might lead to cell-cycle arrest. CldU produces sister-chromatid exchange but has less response to ionizing radiation compared to other thymine analogs. 5-Chloro-2′-deoxyuridine (CldU) is used to study the miscoding potential of hypochlorous acid damage to DNA and DNA precursors. When used with antibody based immunofluorescent imaging, 5-Chloro-2′-deoxyuridine incorporation may be used in protocols to identify sites of DNA replication. CldU may be used as a labeling substrate in conjunction with other halogenated uridine labeling substrates such as iododeoxyuridine (IdU). |
| Packaging: | 100 mg in poly bottle |
| Symbol |   GHS07,GHS08 |
| Signal word | Warning |
| Hazard statements | H302 + H312 + H332 - H351 |
| Precautionary statements | P202 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 20/21/22-40 |
| Safety Statements | 36/37/39-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41106305 |