Anti-CENP-A antibody produced in rabbit
SIGMA/C6867 - ~2 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CenH3; Anti-Centromere Protein A
MDL Number: MFCD08705446
Product Type: Chemical
Immunoblotting
Whole cell lysates were separated on SDS-PAGE, blotted with Anti-CENP-A (Cat. No. C6867) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:2,000. (Lane 1) HEK293 cells (untransfected); (Lane 2, 3) HEK293 cells transfected with human CENP-A; (Lane 3) Inhibition with CENP-A immunizing peptide (20 μg/mL).
Whole cell lysates were separated on SDS-PAGE, blotted with Anti-CENP-A (Cat. No. C6867) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:2,000. (Lane 1) HEK293 cells (untransfected); (Lane 2, 3) HEK293 cells transfected with human CENP-A; (Lane 3) Inhibition with CENP-A immunizing peptide (20 μg/mL).
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-CENP-A antibody produced in Rabbit (Cat. No. C6867) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing CENP-A (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:2,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 17 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293 cells expressing human CENP-A |
| UniProt accession no. | P49450  |
| Application: | Anti-CENP-A antibody has been used in immunoblotting. |
| Application: | Anti-CENP-A antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using HEK-293 cells expressing human CENP-A. |
| Biochem/physiol Actions: | The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of (CENP-A) plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CENP-A is a 17 kDa histone H3- like protein. |
| General description: | Centromere protein A is a protein encoded by the CENPA gene in humans. It is referred as CenH3 and CENP-A. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on a chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin. |
| Immunogen: | synthetic peptide corresponding to amino acids 32-49 located near the N-terminus of human CENP-A, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |