Monoclonal Anti-CNPase antibody produced in mouse
SIGMA/C5922 - clone 11-5B, ascites fluid
Synonym: Anti-CNP1; Anti-HLD20
MDL Number: MFCD00164572
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells. By: Abraham, A. B., Bronstein, R., et al. in Proteome Sci, 2013. PubMed ID: 23621913 Image collected and cropped by CiteAb from the following publication, (http://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-11-18), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells. By: Abraham, A. B., Bronstein, R., et al. in Proteome Sci, 2013. PubMed ID: 23621913 Image collected and cropped by CiteAb from the following publication, (http://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-11-18), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Bovine brain extract was separated on SDS-PAGE and probed with Monoclonal AB To CNP-ASE (MO) CL:11-5B (RB) (Cat. No. C5922). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/1000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 11-5B, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | guinea pig, chicken |
| species reactivity | mouse, rat, bovine, canine, pig, human, rabbit, sheep |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable using brain sections |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:500 using fresh bovine brain extract | |
| UniProt accession no. | P09543  |
| Application: | Monoclonal Anti-CNPase may be used for the localization of CNPase using various immunochemical assays such as ELISA, immunoblot, dot blot and immunocytochemistry. It has also been used in immunohistochemistry. |
| Biochem/physiol Actions: | Monoclonal Anti-CNPase reacts specifically with CNPase in ELISA, immunoblotting and immunohistochemical staining of brain sections. In an immunoblotting assay, the antibody localizes both CNP1 (46kD) and CNP2 (48kD) bands of the enzyme and recognizes whole brain CNPase of human, bovine, mouse, rat, rabbit, dog, sheep and pig but not guinea pig or chicken. Immunohistochemical staining performed on paraffin, cryostat, or vibratome sections of rat brain, reveals a selective staining of oligodendrocytes in the grey and white matter. Nerve cells and axons are not stained and astroglial cells do not appear to be labeled. |
| Biochem/physiol Actions: | The antibody reacts specifically with CNPase in oligodendrocytes and Schwann cells. Recognizes whole brain CNPase. Nerve cells, axons, and astroglial cells do not appear to be labeled. Using immunoblotting techniques, the antibody localizes both CNP1 and CNP2 bands of the enzyme. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase, E.C.3.1.4. 37) is a unique enzyme. It is a constituent of cells that elaborate myelin in the central and peripheral nervous systems, i.e. oligodendrocytes and Schwann cells respectively, and is virtually absent in other cell types in the nervous system. The enzyme isolated from mammalian brain is primarily a mixed dimer of approximately 94 kDa. The dimer consists of a varied proportion of CNP1 (46 kDa) and CNP2 (48 kDa) subunits in various species. The high levels of CNPase observed in oligodendrocytes and Schwann cells portend a vital role of this enzyme in the normal function of these cells. They are distinguished from nearly all other cells by their ability to synthesize and maintain vast amounts of multilamellar membrane, known as myelin. It seems very likely that CNPase is expressed at high levels in these particular cells to facilitate the elaboration and maintenance of myelin or to carry out functions imposed or afforded by the unique membrane structure of myelin. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodefi ciency with CNS involvement, peripheral neuropathies, etc. Another important use is the study of reinnervation of the neuromuscular junction and the identification of oligodendrocyte progenitor cells, very early in postnatal development. |
| Immunogen: | human 2′,3′-cyclic nucleotide-3′-phosphodies |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |