Chlorophyll a
SIGMA/C5753 - from spinach
CAS Number: 479-61-8
Empirical Formula (Hill Notation): C55H72MgN4O5
Molecular Weight: 893.49
EC Number: 207-536-6
MDL Number: MFCD00079050
Linear Formula: C55H72MgN4O5
Product Type: Chemical
Representative of vacuum-sealed clear ampule packaging. Non-uniform tips are an inherent feature in this sealing process.
| ε (extinction coefficient) | 10.6-11.8 × 104 at 428 nm in diethyl ether (vs. 11.17 × 104, lit.) |
| 8.2-9.1 × 104 at 662 nm in diethyl ether (vs. 8.63 × 104, lit.) | |
| assay | ≥85% (HPLC) |
| biological source | spinach |
| form | powder |
| impurities | chlorophyll b, essentially free |
| InChI | 1S/C55H73N4O5.Mg/c1-13-39 |
| InChI key | ATNHDLDRLWWWCB-AENOIHSZSA |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | CCc1c(C)c2cc3c(C=C)c(C)c4 |
| storage temp. | −20°C |
| Application: | A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb). |
| Application: | Chlorophyll a has been used: • to study the variation in carotenoids composition with changing colors and maturation in soybean seeds • to examine the use of chlorophyll distribution maps obtained from hyperspectral imaging for the non-destructive diagnosis of Nitrogen deficiency • as a standard to determine the concentration of the analyte by fluorescence spectrophotometric technique in the water sample collected from the yellow river • as a standard to quantify the amount of chlorophyllous pigments gathered from algae by high performance liquid chromatographic (HPLC) technique • in the identification of pheophytin a from environmentally friendly cultivated green peppers • to sense different nitric oxide (NO) species |
| Biochem/physiol Actions: | Chlorophyll a binds to the protein molecules CP47 and CP43, forming the inner antenna of the reaction center of Photosystem II. In photosystem I, the binding sites of chlorophyll a includes the polypeptides spread across the membranes. Scarcity in formation of chlorophyll and a low chlorophyll density can lead to deficiency of nitrogen in plants leaves. |
| General description: | Chlorophyll a is an important light-harvesting, green or blue color pigment present in the photosynthetic membranes of the chloroplasts in plant cells. It functions as an antenna pigment in photosystems I and II. |
| Packaging: | 1, 5, 10 mg in ampule |
| Packaging: | Sealed ampule. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12171506 |