Calcium chloride
SIGMA/C5670 - anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
Synonym: CaCl2; Calcium dichloride
CAS Number: 10043-52-4
Empirical Formula (Hill Notation): CaCl2
Molecular Weight: 110.98
EC Number: 233-140-8
MDL Number: MFCD00010903
Linear Formula: CaCl2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: C5670-100G
This image depicts SKU: C5670-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: C5670-500G
This image depicts SKU: C5670-500G
| assay | ≥96.0% |
| form | powder |
| grade | anhydrous |
| InChI | 1S/Ca.2ClH/h;2*1H/q+2;;/p |
| InChI key | UXVMQQNJUSDDNG-UHFFFAOYSA |
| mp | 772 °C (lit.) |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | [Cl-].[Cl-].[Ca++] |
| technique(s) | cell culture | insect: suitable |
| cell culture | plant: suitable | |
| vapor pressure | 0.01 mmHg ( 20 °C) |
| Application: | This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published. |
| General description: | Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10 |
| Packaging: | 100, 500 g in poly bottle |
| Preparation Note: | This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 22-24 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥96.0% |
| mp | 772 °C (lit.) |
| UNSPSC | 12352207 |