Cytochrome c Oxidase from bovine heart
SIGMA/C5499 - 5 mg protein/mL
Synonym: EC 1.9.3.1; Ferrocytochrome-
CAS Number: 9001-16-5
EC Number: 232-586-0
MDL Number: MFCD00130899
Product Type: Chemical
Unit Definition One unit of cytochrome c oxidase will oxidize 1.0 μmole of ferrocytochrome c per min. at 25 °C at pH 6.0.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| biological source | bovine heart |
| concentration | 5 mg protein/mL |
| form | buffered aqueous solution |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | 300-350 kDa by gel filtration |
| Quality Level | 200 |
| shipped in | dry ice |
| specific activity | ≥20 units/mg protein |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| Application: | Cytochrome c Oxidase (CcO) from bovine heart has been used:• to study the effect of oxidized glutathione (GSSG) on the number of total free cysteines • in a histochemical assay to measure CcO activity in tissue sections • to study the redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy |
| Biochem/physiol Actions: | Cytochrome c oxidase is the principal terminal oxidase of high oxygen affinity in the aerobic metabolism of all animals, plants, yeasts and some bacteria. |
| Biochem/physiol Actions: | Cytochrome c oxidase is the principal terminal oxidase of high oxygen affinity in the aerobic metabolism of all animals, plants, yeasts and some bacteria. It is present in the mitochondria of the more highly developed cells and in the cytoplasmic membrane of bacteria. Cytochrome c oxidase catalyses the electron transfer from cytochrome c to O2. This electron-transfer process produces a proton gradient across the membrane, which in turn drives the production of ATP. This enzyme is unique in providing energy for the cell bycoupling the electron transport through the cytochrome chain with the process of oxidative phosphorylation. |
| General description: | Research area: Cell cycle Cytochrome c oxidase contains 13 subunits, encoded by mitochondrial genes and nuclear genome. It is comprised of two heme groups (Fea and Fea3) and two copper centers, CuA and CuB. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles . |
| Other Notes: | One unit of cytochrome c oxidase will oxidize 1.0 μmole of ferrocytochrome c per min. at 25 °C at pH 6.0. |
| Physical form: | Supplied as a solution in 25 mM Tris-HCl buffer, pH 7.8, 5 mM EDTA, and 39 mM n-dodecyl β·-D-maltoside. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.9.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |