Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse
SIGMA/C5301 - clone M20, ascites fluid
Synonym: Anti-CARD2; Anti-CK-8; Anti-CK8; Anti-CYK8; Anti-K2C8; Anti-K8; Anti-KO
MDL Number: MFCD00162254
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 1:200 Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in Mouse, Clone: M20 (Cat. No. C5301) followed by 1:20 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M20, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 52 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | feline, human, rabbit, canine, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): suitable |
| indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue | |
| western blot: suitable | |
| UniProt accession no. | P05787  |
| Application: | Monoclonal anti-cytokeratin peptide 8 antibody has been used in • immunofluorescence microscopy • immunohistochemistry • dot blotting • protein preparation from bovine epithelial cells • immunofluorescence • western blotting |
| Biochem/physiol Actions: | Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. |
| Biochem/physiol Actions: | Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.Monoclonal Anti-Cytokeratin 8 (mouse IgG1 isotype) is derived from the M20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from the human breast carcinoma cell line MCF7. |
| Immunogen: | cytokeratin from human breast carcinoma cell line MCF7. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |