Calmodulin bovine
SIGMA/C4874 - recombinant, expressed in E. coli, lyophilized powder, ≥98% (SDS-PAGE)
Synonym: CaM, Phosphodiesterase 3:5-cyclic nucleotide activator; Phosphodiesterase 3′:5′-cyclic nucleotide activator
MDL Number: MFCD00131869
Product Type: Chemical
SDS-PAGE Purity of Calmodulin Bovine (Cat. No. C4874) was assessed on SDS-PAGE. Lanes: 1. 1 μg/mL 2. 0.5 μg/mL
| assay | ≥98% (SDS-PAGE) |
| biological source | bovine |
| composition | Protein, ≥85% |
| form | lyophilized powder |
| mol wt | Mw 19000.9 by amino acid sequence |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| UniProt accession no. | P62157  |
| Application: | Calmodulin bovine has been used inin vitro phosphorylation assay of recombinant retinoic acid-inducible gene I protein. It has also been used in the endoprotease Glu-C proteolysis and deamidation studies. |
| Biochem/physiol Actions: | Ca2+ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP-ribose and is involved in intracellular Ca2+ homeostasis. |
| Biochem/physiol Actions: | Calmodulin from bovine undergoes conformational changes upon calcium binding. It binds to sphingosylphosphorylcholi |
| General description: | Calmodulin from bovine takes up a dumb-bell-structure. Two calcium binding EF hand loops, antiparallel β-sheet and three α-helices comprises a lobe. It has a central helix connecting the lobes. Calmodulin can bind four calcium molecules in a cooperative interaction pattern. |
| General description: | Sequence: MGSSHHHHHHSSGLVPRGSHMADQL |
| Preparation Note: | Produced using animal component-free materials. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |