Monoclonal Anti-Cathepsin L antibody produced in mouse
SIGMA/C4618 - clone CPL33/1, purified from hybridoma cell culture
Synonym: Anti-CATL; Anti-CTSL; Anti-MEP
MDL Number: MFCD01321805
Product Type: Chemical
Immunoblotting Equal amounts of total cell extract of A549 cells were separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L (Cat. No. C4618). The antibody was developed with Goat Anti-Mouse – Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate Lanes 1: 1 μg/mL antibody 2: 0.5 μg/mL 3: 0.25 μg/mL 4: 0.12 μg/mL 5: Negative control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CPL33/1, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~25 kDa (human cathepsin L) |
| antigen ~42 kDa (human pro cathepsin L) | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 0.1-0.2 μg/mL using total cell extracts of A549 cells |
| Application: | Anti-Cathepsin L antibody has been used for immunoblotting. |
| Application: | Monoclonal Anti-Cathepsin L antibody produced in mouse is suitable for: • immunohistochemistry • indirect ELISA • western blot : 0.1-0.2 μg/mL using total cell extracts of A549 cells |
| Biochem/physiol Actions: | Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. |
| Biochem/physiol Actions: | Inhibition of the enzyme or the proenzyme by low molecular weight inhibitors or by specific antibodies led to a suppression of the invasive capabilities of malignant cells, or a decline in their ability to form tumors in experimental in vivo and in vitro models. |
| Biochem/physiol Actions: | The antibody recognizes the native and denaturated forms of the protein and does not cross react with human cathepsin V. Monoclonal Anti-Cathepsin L specifically recognizes human cathepsin L (∼ 25 kDa) and procathepsin L (∼ 42 kDa). Anti-Cathepsin L antibody epitope resides within amino acids of human cathepsin L (FYKE). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Cathepsin L antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human procathepsin L. |
| General description: | Monoclonal Anti-Cathepsin L (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from BALB/c mice immunized with human procathepsin L. |
| Immunogen: | human procathepsin L. The antibody epitope resides within amino acids 258-261 of human cathepsin L (FYKE). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |