Anti-Caveolin-1 antibody produced in rabbit
SIGMA/C4490 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Caveolin-1; Anti-VIP21
MDL Number: MFCD01633097
Product Type: Chemical
Immunofluorescence
Plasma Membrane Caveoli Staining: HUVEC cells were fixed with 4% paraformaldehyde/0.1% Triton® X-100 and stained with Anti-Caveolin-1 (Cat. No. C4490) at a 1:100 dilution, followed by Goat Anti-Rabbit IgG-FITC (Cat. No. F9887)
Plasma Membrane Caveoli Staining: HUVEC cells were fixed with 4% paraformaldehyde/0.1% Triton® X-100 and stained with Anti-Caveolin-1 (Cat. No. C4490) at a 1:100 dilution, followed by Goat Anti-Rabbit IgG-FITC (Cat. No. F9887)
Immunoblotting
NIH3T3 (Lanes A, C) and ECV304 whole cell extracts (Lane B) were separated on SDS-PAGE, blotted with Rabbit Anti-Caveolin-1(Cat. No. C4490) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:2,000. Specificity of antibody is confirmed by inhibition of staining with the Caveolin-1 immunizing peptide (5 μg/mL, Lane C).
NIH3T3 (Lanes A, C) and ECV304 whole cell extracts (Lane B) were separated on SDS-PAGE, blotted with Rabbit Anti-Caveolin-1(Cat. No. C4490) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:2,000. Specificity of antibody is confirmed by inhibition of staining with the Caveolin-1 immunizing peptide (5 μg/mL, Lane C).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 22 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 4-8 μg/mL using HUVEC cell line |
| indirect immunofluorescence: suitable | |
| western blot: 1-2 μg/mL using whole cell extract of the human endothelial ECV304 cell line and the mouse fibroblast NIH3T3 cell line | |
| UniProt accession no. | Q03135  |
| Application: | Anti-Caveolin-1 antibody produced in rabbit has been used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Caveolin can simultaneously recognize GPI-linked proteins and interact directly with a number of caveolae associated downstream signaling molecules, such as H-Ras, hetero-trimeric G-proteins, annexin-II, epidermal growth factor receptor (EGFR), protein kinase C, src-family tyrosine kinases and nitric oxide synthase (NOS) isoforms. Caveolin-1 participates in angiogenesis and is expected to play a major role in the growth and evolution of cancer. It participates in glucose metabolism by controlling the expression of glucose transporters. |
| Biochem/physiol Actions: | The sequence is highly conserved in many species, e.g., identical in rat, mouse, bovine, and dog caveolin-1. The sequence is not found in caveolin-2 or caveolin-3. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Caveolin, a 20-24 kDa integral transmembrane protein, has been identified as a principal component of caveolae membranes. Caveolin (also termed VIP21) exists in several isoforms termed caveolin-1, caveolin-2 and caveolin-3. Caveolin-1 (CAV1) (20- 22 kDa) can exist as two isoforms, caveolin-1α and -1β due to alternative splicing of the mRNA. Caveolin-1 and -2 have similar tissue distribution. |
| Immunogen: | synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 (amino acids 2-20). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |