Collagen from human placenta
SIGMA/C4407 - Bornstein and Traub Type III (Sigma Type X), powder
CAS Number: 9007-34-5
EC Number: 232-697-4
MDL Number: MFCD00130825
Product Type: Chemical
SDS-PAGE Purity of Collagen from Human placenta (Cat. No. C4407) was assessed on SDS-PAGE.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4407-25MG
| assay | ≥90% (GE) |
| biological source | human placenta |
| form | powder |
| impurities | HIV, hepatitis B and hepatitis C, none detected |
| Quality Level | 200  |
| solubility | aqueous acid: soluble |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | Q03692  |
| Analysis Note: | An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands. |
| Application: | Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens. |
| Disclaimer: | This product should be stored at 2-8 °C and will retain activity in these conditions for 2 years. |
| Other Notes: | All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. |
| Other Notes: | Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types. |
| Packaging: | 1, 5, 25 mg in glass bottle |
| Preparation Note: | This product was prepared by a modification of the pepsin extraction and salt fractionation method of Hill, R.J. and Harper, E., Anal. Biochem., 141 (1984). It is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (GE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |