Butyrylcholinesterase from equine serum
SIGMA/C4290 - lyophilized powder, ≥500 units/mg protein
Synonym: Acylcholine acyl-hydrolase; Choline esterase, butyryl; Pseudocholinesterase
CAS Number: 9001-08-5
EC Number: 232-579-2
MDL Number: MFCD00081455
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the simple uncoupled assay using acetylcholine which can be measured titrimetrically.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using DTNB detection.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using ferricyanate detection.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4290-1KU
| biological source | equine serum |
| composition | Protein, ≥10% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥500 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret |
| Application: | Butyrylcholinesterase (BChE) from equine serum has been used: • to determine the inhibitory concentration of bupivacaine on butyrylcholinesterase • in acetylcholinesterase (AChE)/BChE activity assay to determine the inhibitory activity of benzothiazole-piperazine compounds |
| Application: | Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity shows promise. There is a correlation between the level of BChE in human blood and degree of protection against potentially toxic nerve agents. There has also been interest in the roles of cholinesterases with regard to Alzheimer’s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. The enzyme has been used to test bupivacaine as an inhibitor of butyrylcholinesterase during acetylcholinesterase assay using cerebrospinal fluid. |
| Biochem/physiol Actions: | Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca2+ and Mg2+ and the activity is constant over the pH range 6.0-8.0. It is inhibited by Betaine, nicotine, organophosphates, carbamates. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine. |
| Packaging: | 1000 units in poly bottle |
| Physical form: | Highly purified, lyophilized powder containing buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥500 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.8 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |