Cystatin B human
SIGMA/C4249 - recombinant, expressed in E. coli
Synonym: CPI-B; CST6; CSTB; EPM1; Liver Thiol Proteinase Inhibitor; PME; STFB; Stefin B
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥95% (SDS-PAGE) |
| description | contains a N-terminal 7X His-tag |
| form | solid |
| mol wt | apparent mol wt ~13 kDa by SDS-PAGE (reducing) |
| NCBI accession no. | NP_000091   |
| Quality Level | 200 |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| Application: | Suitable for use as a cysteine-protease inhibitor. Is tested for suitability as an inhibitor of the enzymatic activity of papain. |
| Biochem/physiol Actions: | Cystatin B was shown to interact with the protein RACK-1, β-spectrin and neurofilament light chain NF-L forming a unique multiprotein complex specifically in the cerebellum, confirming its function in the brain. |
| General description: | Cystatin B is a member of the type 1 (stefins) superfamily of cystatins. Cystatin B is encoded by the CSTB gene and functions as an intracellular thiol protease inhibitor regulating the activity of members of the papain family and cathepsins B, H, and L. Cystatin B may also protect cellular components from the effects of proteases that leak from lysosomes. Mutations in the CSTB gene are thought to be the primary defect in patients with Progressive Myoclonus Epilepsy (EPM1). |
| Preparation Note: | Cystatin B should be reconstituted at 100 μg/mL in steile 25 mM Tris pH 7.5 and 100 mM NaCl. Reconstituted product should be stored in single-use aliquots at -70° C and should be stable for at least 3 months. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |