Protamine Kinase, Cytosolic from bovine kidney
SIGMA/C4237 - >90% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: Cytosolic Protamine Kinase
MDL Number: MFCD04041070
Product Type: Chemical
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| assay | >90% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | 45 kDa |
| packaging | vial of 100 ng |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥15,000 units/mg protein |
| storage temp. | −70°C |
| Application: | Cytosolic Protamine Kinase (cPK) is isolated from bovine kidney and has a molecular mass of approximately 45 kDa. It phosphorylates the eukaryotic Initiation Factor 4E (eIF4E), which initiates translation by mRNA. It is used to study protein synthesis and various cellular processes. |
| Biochem/physiol Actions: | Cytosolic protamine kinase is involved in the regulation of protein synthesis and is indirectly associated with numerous cellular processes. Cytosolic protamine kinase is a distinct insulin-stimulated kinase involved in the phosphorylation of eukaryotic Initiation Factor 4E (eIF4E) which is key to initiating translation by mRNA. This protein appears to be inactivated by protein phosphatase 2A family members and may also be inhibited by microcystin, okadeic acid, and ATP. The phosphorylation process is reversible and MBPK1 and MBPK2 (Myelin Basic Protein Kinase 1 and 2) may reactivate cytosolic protamine kinase . |
| Other Notes: | One unit will incorporate 1 nmol of phosphate using protamine sulfate as a substrate per minute at pH 7.0 at 30 °C. |
| Physical form: | Solution in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, 50% glycerol |
| WGK Germany | 1 |
| Purity | >90% (SDS-PAGE) |
| activity | specific activity: ≥15,000 units/mg protein |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |