Anti-phospho-β-Catenin (pSer33/pSer37) antibody, Mouse monoclonal
SIGMA/C4231 - clone BC-22, purified from hybridoma cell culture
Synonym: Anti-CTNNB; Anti-EVR7; Anti-MRD19; Anti-NEDSDV; Anti-armadillo
MDL Number: MFCD03457566
Product Type: Chemical
Immunoblotting Whole lysate of HEK-293T cells treated with proteasome inhibitor MG132 was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-β-Catenin (pSer33/pSer37), Clone: BC-22 (Cat. No. C4231). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 3.5 μg/mL 2. Negative control: without first antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BC-22, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~94 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat, chicken |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer33/pSer37) |
| technique(s) | indirect ELISA: suitable |
| microarray: suitable | |
| western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132 | |
| UniProt accession no. | P35222  |
| Application: | Monoclonal Anti-phospho-ß-Catenin (pSer33/pSer37) antibody is suitable for: • indirect ELISA • microarray • western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132. |
| Biochem/physiol Actions: | β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target. In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression. |
| Biochem/physiol Actions: | The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphosphorylated peptide. It does not detect the unphosphorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus. |
| Immunogen: | synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |