Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)
SIGMA/C4018 - lyophilized powder, ≥100 units/mg protein
Synonym: 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
CAS Number: 37289-07-9
MDL Number: MFCD00130789
Product Type: Chemical
Unit Definition One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4018-3KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C4018-7.5KU
| composition | Protein, ≥50% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥100 units/mg protein |
| storage temp. | −20°C |
| Application: | The enzyme from Sigma has been used in the analysis of bile samples in various studies. |
| Biochem/physiol Actions: | The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C. |
| Packaging: | 1500 units in glass bottle |
| Packaging: | 3000, 7500 units in poly bottle |
| Physical form: | Partially purified lyophilized powder containing buffer salts and stabilizer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.1.24 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |