Carbonic Anhydrase from bovine erythrocytes
SIGMA/C3934 - lyophilized powder, ≥2,000 W-A units/mg protein
Synonym: Carbonate Dehydratase; Carbonate Hydrolyase
CAS Number: 9001-03-0
EC Number: 232-576-6
MDL Number: MFCD00081467
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3934-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3934-500MG
| application(s) | diagnostic assay manufacturing |
| biological source | bovine erythrocytes |
| form | lyophilized powder |
| mol wt | 30 kDa |
| Quality Level | 300  |
| specific activity | ≥2,000 W-A units/mg protein |
| storage temp. | 2-8°C |
| Application: | Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA. |
| Biochem/physiol Actions: | Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson’s disease. |
| Other Notes: | One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.) |
| Packaging: | 100, 500 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2,000 W-A units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 4.2.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |