Collagen, Type I solution from rat tail
SIGMA/C3867 - BioReagent, suitable for cell culture, sterile-filtered
Synonym: Collagen from rat tail; Col1a1; Collagen from rat tail
CAS Number: 9007-34-5
EC Number: 232-697-4
MDL Number: MFCD00130825
Product Type: Chemical
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| assay | >95% (SDS-PAGE) |
| binding specificity | Peptide Source: Collagen |
| Peptide Source: Fibronectin |
|
| biological source | rat tail |
| concentration | >0.5 mg/mL Biuret |
| description | Approx. 100mg/vial ((35 - 40 ml) ) |
| form | liquid |
| impurities | Mycoplasma Test by Barile, none detected |
| mol wt | apparent mol wt 115-130 kDa by SDS-PAGE (doublet) |
| apparent mol wt 215-235 kDa by SDS-PAGE (doublet) | |
| NCBI accession no. |   |
| packaging | pkg of 1 vial |
| product line | BioReagent |
| Quality Level | 200 |
| shipped in | wet ice |
| solubility | soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid) |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| surface coverage | 6‑10 μg/cm2 |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02454 |
| Application: | Collagen, Type I solution from rat tail has been used for coating culture plates and dishes for human hepatocyte HepG2 cell line and podocytes. It has also been used as a standard for total collagen content calibration curve generation for the quantification of liver extracellular matrix pre-gel. |
| Application: | Used as a coating material to support adherent cells growth and differentiation. |
| Biochem/physiol Actions: | Collagen protein is essential for the mechanical integrity of tendons and bone. Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes. |
| Features and Benefits: | • Sourced from rat tail. • Sterile-filtered to minimize contamination risks. • Suitable for a wide range of cell culture applications. • Provides optimal surface coverage (6-10 μg/cm2) for promoting cell adhesion and proliferation. |
| General description: | Collagen is an extracellular matrix protein comprising triple helical structure. The structure of collagen type I, in particular, is characterized by the presence of three identical α-chains in its homotrimetric form. Among these three α-chains, one exhibits slight sequence variation. Type I collagen was first isolated from rat-tail tendon, has two main α chains (α and α ) and one β chain. |
| Other Notes: | Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types. |
| Other Notes: | When C3867 is first removed from the cooler it will appear gelled. When it is allowed to come fully to 37°C it will appear as a liquid. |
| Preparation Note: | Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation. |
| Preparation Note: | Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2. SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |