Creatine Phosphokinase from rabbit muscle
SIGMA/C3755 - Type I, salt-free, lyophilized powder, ≥150 units/mg protein
Synonym: ATP: Creatine N-Phosphotransferase; CPK; Creatine Kinase; Phosphocreatine phosphokinase
CAS Number: 9001-15-4
EC Number: 232-585-5
MDL Number: MFCD00130876
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3755-17.5KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C3755-35KU
| foreign activity | ATPase ≤0.01% |
| Lactic dehydrogenase, hexokinase, myokinase and pyruvate kinase ≤0.001% | |
| form | salt-free, lyophilized powder |
| mol wt | 80-86.2 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| solubility | 0.25 M glycyl-glycine, pH 7.4: soluble 5.0 mg/mL, clear, colorless to slightly yellow |
| specific activity | ≥150 units/mg protein |
| storage temp. | −20°C |
| type | Type I |
| Analysis Note: | Protein determined by biuret. |
| Application: | Molecular Weight: ~81,000 Creatine Phosphokinase is a dimer composed predominantly of the skeletal muscle derived homodimer (MM). CK also exists as a heterodimer (MB) particularly in the myocardium. CK derived from brain tissue consists mainly of the brain source homodimer (BB). The amino acid sequences of the M chain and B chains are about 80% homologous. From the sequence, the molecular weight of the M chain is 43,112. E1%(280)= 8.76 pH Optimum: pH 8.8-9.0 for the forward reaction and pH 6.0-7.0 for the reverse reaction. CK is a cellular enzyme with a wide tissue distribution. Its physiological role is associated with ATP generation for contractile or transport systems. Increased levels of CK are associated with myocardial infarction, muscular dystrophy, hyperthyroidism, pulmonary infarction and cerebrovascular disease. Variations in relative isozyme distribution can provide additional information in the diagnosis of these conditions. Substrates: Creatine, N-ethylglycocyamine and glyocyamine have been shown to act as substrates for CK. CK is very specific for ATP/ADP. Inhibitors: ADP is a strong inhibitor of the forward reaction competing with ATP. Divalent cations such as Ca2+ (Ki=4.5 mM), Zn2+ and Cu2+ inhibit CK by competing with Mg2+. Other inhibitors include acetate, acetylsalicylic acid, adenosine, p-aminosalicylic acid, AMP, benzoic acid, bicarbonate, bromide, chloride, p-Chloromercuribenzoic acid, ethylene oxide, 2,4-fluorodinitrobenzene, iodide, malonic acid, NAD, nitrate, phosphate, pyrophosphate, salicylic acid, sulfate, sulfite, thyroxine, trichloroacetate, L-triiodothyroxine, L-triiodothyronine, and tripolyphosphate. |
| Application: | The enzyme from Sigma has been used in the measurement of phosphocreatine in hippocampal neurons isolated from rat brains. |
| Disclaimer: | The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution. |
| Other Notes: | One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C. |
| Packaging: | 500, 1000, 3500, 17500, 35000 units in poly bottle |
| Preparation Note: | Produces a clear, colorless to light yellow solution at 5 mg/mL in 0.25 M glycyl-glycine, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥150 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.3.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |