Anti-Pan Cadherin antibody produced in rabbit
SIGMA/C3678 - whole antiserum
Synonym: Anti-ACOGS; Anti-ARVD14; Anti-CD325; Anti-CDHN; Anti-CDw325; Anti-NCAD
MDL Number: MFCD00162729
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Phenotypic chemical screening using a zebrafish neural crest EMT reporter identifies retinoic acid as an inhibitor of epithelial morphogenesis. By: Jiménez, L., Wang, J., et al. in Dis Model Mech, 2016. PubMed ID: 26794130 Image collected and cropped by CiteAb from the following publication, (http://dmm.biologists.org/lookup/doi/10.1242/dmm.021790), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), Monoclonal Anti-Human Vinculin, clone No. hVIN-1 (Cat. No. V9131) (red) and Rabbit Anti-Pan Cadherin (Cat. No. C3678) (blue). Focal contacts appear yellow and intercellular junctions white.
From Prof. B. Geiger, Prof. Z. Kam and T. Volberg, Weizmann Institute of Science, Rehovot, Israel.
A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), Monoclonal Anti-Human Vinculin, clone No. hVIN-1 (Cat. No. V9131) (red) and Rabbit Anti-Pan Cadherin (Cat. No. C3678) (blue). Focal contacts appear yellow and intercellular junctions white.
From Prof. B. Geiger, Prof. Z. Kam and T. Volberg, Weizmann Institute of Science, Rehovot, Israel.
Immunohistochemistry
Immunoperoxidase staining of a formalin-fixed, paraffin-embedded section of human heart using Rabbit Anti-Pan Cadherin (Cat. No. C3678).
Immunoperoxidase staining of a formalin-fixed, paraffin-embedded section of human heart using Rabbit Anti-Pan Cadherin (Cat. No. C3678).
Western Blotting Whole extract of Chicken Cardiac Muscle were separated on SDS-PAGE and probed with Anti-Pan Cadherin antibody produced in Rabbit (Cat. No. C3678). The antibody was developed using 1:1,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:200 antibody 2. 1:400 antibody 3. Negative Control
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen 135 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, cat, chicken, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using protease-digested animal heart sections |
| indirect immunofluorescence: 1:100 using cultured MDBK cells | |
| western blot: 1:200 | |
| UniProt accession no. | P19022  |
| Application: | Anti-Pan Cadherin antibody is suitable for use in confocal immunofluorescence using rat ventricular myocytes and western blot (1:200). The antibody may also be used for indirect immunofluorescence (1:100 using cultured MDBK cells) and immunohistochemistry (in formalin-fixed, paraffin-embedded sections or at 1:1,000 dilutions using protease-digested animal heart sections). Additionally, the product can be used in indirect immunoblot (135 kDa band in chicken or rabbit heart extracts), immunoperoxidase and immunoelectron-microscopy assays. |
| Application: | May be used for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type. |
| Biochem/physiol Actions: | The epitope recognized by the antibody is shared by a wide variety of organisms. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cadherins are glycoprotein receptors that regulate Ca2+-dependant adhesion in cells. Cadherins are associated with adherens junctions and are mainly involved in morphogenesis of cells and tissues. P-cadherin is expressed in epithelial cells and its over-expression has been associated with breast cancer metastasis and invasion . Anti-Pan Cadherin antibody is specific for cadherins in a wide range of organisms including chickens, rabbits and rats. |
| Immunogen: | Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled with glutaraldehyde to keyhole limpet hemocyanin (KLH). |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |