Anti-Connexin-32 (265-279) antibody produced in rabbit
SIGMA/C3470 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CMTX; Anti-CMTX1; Anti-CX32
MDL Number: MFCD01633267
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Rat liver were stained with 1:400 Anti-Connexin-32 (265-279) antibody produced in Rabbit (Cat. No. C3470), followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting Rat liver extract was separated on SDS-PAGE and probed with APA to Connexin 32 (265-279) (RB) (Cat. No. C3470). The antibody was developed with Alkaline Phosphatase conjugate (Cat. No. A9919) and a NBT/BCIP substrate. Antibody concentration 1/1,200
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 27 kDa |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1:600 using rat liver tissue |
| microarray: suitable | |
| western blot: 1:600 using a rat liver membrane preparation | |
| UniProt accession no. | P08034   |
| Application: | Anti-Connexin-32 (265-279) antibody produced in rabbit has been used in western blot analysis and immunocytochemistry. |
| Biochem/physiol Actions: | Mutations of the gap junction protein β 1 (GJB1) gene results in the X-linked form of Charcot-Marie-tooth disease (CMTX1). Cx32 participates in the liver regeneration after partial hepatectomy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Connexins (Cx) are a multi-gene family of highly related proteins with molecular weights ranging from 26 to 70 kD. The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail of varying length. Anti-Connexin 32 is developed in rabbit using synthetic peptide Lys- Arg- Ser-Pro- Gly- Thr- Gly- Ala-Gly- Leu- Ala- Glu- Lys-Ser- Asp- Arg conjugated to KLH with glutaraldehyde as immunogen. |
| General description: | Gap junction protein, connexin-32 (Cx32) is encoded by gap junction protein β 1 (GJB1) gene, localized on the human chromosome X. This gene is expressed in the central nervous system (CNS) in oligodendrocytes and other neuronal populations. |
| Immunogen: | synthetic human/rat connexin-32 peptide (amino acids 265-279 with an N-terminally added lysine). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |