Citrate Synthase from porcine heart
SIGMA/C3260 - ammonium sulfate suspension, ≥100 units/mg protein
Synonym: Citrate condensing enzyme; Citrate oxaloacetate lyase (CoA-acetylating)
CAS Number: 9027-96-7
MDL Number: MFCD00130808
Product Type: Chemical
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| biological source | Porcine heart |
| foreign activity | isocitrate dehydrogenase and aconitase ≤0.01% |
| malic dehydrogenase ≤0.1% | |
| form | ammonium sulfate suspension |
| mol wt | 98 kDa ( 49 kDa monomer) |
| Quality Level | 200  |
| solubility | H2O: soluble 1.0 mg/mL, clear |
| specific activity | ≥100 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Citrate Synthase from porcine heart has been used: • to inject newt egg for determining its importance in egg activation at fertilization • in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity • to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs |
| Biochem/physiol Actions: | Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine. |
| Other Notes: | One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C. |
| Physical form: | Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0. |
| Preparation Note: | Dissolves in water to form a clear solution at 1 mg/mL concentration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 4.1.3.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |