Chondroitinase ABC from Proteus vulgaris
SIGMA/C2905 - lyophilized powder, 0.3-3 units/mg solid
Synonym: Chondroitin ABC Lyase
CAS Number: 9024-13-9
EC Number: 232-777-9
MDL Number: MFCD00130795
Product Type: Chemical
Chondroitinase A,B and C specificities
Axonal regeneration inhibitors accumulate at the injury site including a family of inhibitors called Chondroitin sulfate proteoglycans (CSPGs). This family of molecules have a core protein with a sulfated glycosaminoglycan (GAG) side chain. When the GAG is digested by Chondroitinase ABC (ChABC) axonal regeneration can occur.
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial (Proteus vulgaris) |
| composition | Protein, ~35% biuret |
| conjugate | (Glucosaminoglycan) |
| form | lyophilized powder |
| mol wt | ~120 kDa by gel filtration |
| Quality Level | 300  |
| specific activity | 0.3-3 units/mg solid |
| storage temp. | −20°C |
| Application: | Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan. |
| Biochem/physiol Actions: | Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuron |
| Other Notes: | One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β- |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 2, 5, 10 units in glass bottle |
| Packaging: | Packages based on chondroitinase C activity. |
| Physical form: | Lyophilized powder containing Tris buffer salts |
| Preparation Note: | Reconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 0.3-3 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.2.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |