Acetylcholinesterase from Electrophorus electricus (electric eel)
SIGMA/C2888 - Type V-S, lyophilized powder, ≥1,000 units/mg protein
Synonym: AChE; Acetylcholine acetylhydrolase; Cholinesterase, Acetyl
CAS Number: 9000-81-1
EC Number: 232-559-3
MDL Number: MFCD00081268
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the simple uncoupled assay using acetylcholine which can be measured titrimetrically.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using DTNB detection.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using ferricyanate detection.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2888-1KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2888-500UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2888-5KU
| biological source | Electrophorus electricus |
| composition | Protein, ≥45% biuret |
| form | lyophilized powder |
| mol wt | 280 kDa |
| pI | ~5.3 |
| Quality Level | 200  |
| solubility | Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5) |
| specific activity | ≥1,000 units/mg protein |
| storage temp. | −20°C |
| type | Type V-S |
| Analysis Note: | The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel. |
| Application: | Acetylcholinesterase from Electrophorus electricus (electric eel) has been used: • in acetylcholinesterase activity assay • to study its effects on Xenopus embryonic hippocampal cells • to study the effect of acetylcholine inhibitors on development and plasticity |
| Biochem/physiol Actions: | Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway. |
| Biochem/physiol Actions: | Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid. |
| Disclaimer: | For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated. |
| General description: | Molecular Weight: 280 kDa Isoelectric Point: 5.5 Extinction Coefficient: E1% = 18.0 (280 nm) Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C. |
| Packaging: | 500, 1000, 5000 units in glass bottle |
| Physical form: | Lyophilized powder containing Tris buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.7 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |