Cytochrome c from equine heart
SIGMA/C2867 - BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture
Synonym: CYC; CytC; Cytochrome c from horse heart
CAS Number: 9007-43-6
EC Number: 232-700-9
MDL Number: MFCD00130890
Product Type: Chemical
Cytochrome c is a heme protein containing a single polypeptide chain and a single heme group, which is covalently attached to Cys14 and Cys17.
SDS-PAGE (4-20%) of cytochrome c (Cat. No. C2867) under reducing conditions and stained with Coomassie blue.
The electron transport protein complex of the inner mitochondrial membrane
HPLC analysis of cytochrome c (Cat. No. C2867) using TFA/ACN gradient on Supelco Discovery® C8 15 cm × 2.1 mm, UV detection at 214 nm.
MALDI-ToF mass spectrometry analysis of a representative lot of cytochrome c (Cat. No. C2867). Results indicate only the three charge states of cytochrome c are detected.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C2867-50MG
| assay | ≥99% (SDS-PAGE) |
| biological source | horse heart |
| form | powder |
| mol wt | 12,384 |
| product line | BioUltra |
| Quality Level | 300  |
| solubility | water: 10 mg/mL, dark red-brown |
| storage condition | (Tightly closed Dry) |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P00004  |
| Application: | Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis. |
| Application: | The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell. |
| Biochem/physiol Actions: | Cytochrome C (Cyt C) acts as a potent signaling molecule of apoptosis by activating caspase 3, a death protease. It plays a role in heme-binding with the help of cytochrome c heme lyase which facilitates its release into the mitochondrial intermembrane space. Cyt C also exhibits lipid-binding by interacting with cardiolipin, a phospholipid that initiates protein unfolding and activates the peroxidase function in Cyt C. |
| Biochem/physiol Actions: | Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen |
| General description: | Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria. Research area: Apoptosis |
| Other Notes: | View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enz. |
| Packaging: | 10, 50 mg in glass bottle |
| Preparation Note: | A further purification of C2506 |
| Preparation Note: | Prepared using TCA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |