Chondroitinase AC from Flavobacterium heparinum
SIGMA/C2780 - lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
Synonym: Chondroitin AC lyase
CAS Number: 9047-57-8
MDL Number: MFCD00130796
Product Type: Chemical
Chondroitinase A,B and C specificities
| composition | Protein, ~15% Lowry |
| conjugate | (Glucosaminoglycan) |
| foreign activity | Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount |
| form | lyophilized powder |
| Quality Level | 200  |
| solubility | 0.02 M phosphate buffer: soluble (pH 7.0) |
| specific activity | 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate) |
| storage temp. | −20°C |
| Application: | Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate. |
| Application: | Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements. |
| Application: | Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides |
| Biochem/physiol Actions: | Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate). |
| Other Notes: | Contains potassium phosphate buffer salts and BSA as stabilizer. |
| Other Notes: | One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm). |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 5 units in serum bottle |
| Preparation Note: | Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.2.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |