Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse
SIGMA/C2562 - clone C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, ascites fluid
Synonym: Monoclonal Anti-Cytokeratin, pan (mixture); Panck Antibody; Panck Antibody - Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse
MDL Number: MFCD00164710
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Deducing the stage of origin of Wilms′ tumours from a developmental series of Wt1-mutant mice. By: Berry, R. L., Ozdemir, D. D., et al. in Dis Model Mech, 2015. PubMed ID: 26035382 Image collected and cropped by CiteAb from the following publication, (http://dmm.biologists.org/cgi/doi/10.1242/dmm.018523), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Deducing the stage of origin of Wilms′ tumours from a developmental series of Wt1-mutant mice. By: Berry, R. L., Ozdemir, D. D., et al. in Dis Model Mech, 2015. PubMed ID: 26035382 Image collected and cropped by CiteAb from the following publication, (http://dmm.biologists.org/cgi/doi/10.1242/dmm.018523), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Fetal rat lung epithelial cells were labeled with Monoclonal Anti-pan-Cytokeratin (mixture) (Cat. No. C2562) (green) and DNA was counterstained with DAPI (blue). From Paulina Piairo, Development & Neoplasia, Biochimiche e Cellulari, University of Minho, Campus de Gualtar, Braga, Portugal.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human skin sections with 1:100 Monoclonal Anti-Cytokeratin, pan (Mixture) (Cat. No. C2562) using Goat Anti-Mouse IgG, FITC-conjugate (Cat. No. F5262).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Cytokeratin, pan (Mixture) (Cat. No. C2562). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. HepG2 3. COS-7
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C-11+PCK-26+CY-90+KS-1A3+ |
| conjugate | unconjugated |
| contains | 7% horse serum and 15 mM sodium azide as preservative |
| isotype | IgG1/IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using protease-digested sections of human or animal tissues |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: 1:100 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues | |
| western blot: suitable |
| Application: | Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse is suitable for the following applications: • Immunohistochemistry (formalin-fixed, paraffin-embedded sections) using protease-digested sections of human or animal tissues. • Immunohistochemistry (frozen sections). • Indirect immunofluorescence (at a working dilution of 1:100 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues). • Immunocytochemical labeling (immunofluorescence ) of cells. • Western blotting. |
| Biochem/physiol Actions: | Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in the epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type. |
| Biochem/physiol Actions: | This is a mixture of monoclonal anti-cytokeratin antibodies. It recognizes human cytokeratins 1,4,5,6,8,10,13,18, and 19. It is a broad spectrum reagent, which reacts specifically with a wide variety of normal, reactive, and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. Increased staining intensity is seen following proteolytic treatment (protease unmasking). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Cytokeratin, pan (mouse IgG1 and IgG2a isotypes) is a mixture of monoclonal antibodies from the following clones: C-11, PCK-26, CY-90, KS1A3, M20, and A53-B/A2. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1 (68 kDa), 4 (59 kDa), 5 (58 kDa), 8 (52 kDa), and 6 (56 kDa) are members of the type II neutral-to-basic subfamily expressed in cornified epithelia, non-cornified stratified squamous epithelia, stratified epithelia, simple epithelia and in tissues with natural or pathological turnover respectively. Cytokeratins 10 (56 kDa), 13 (54 kDa, and 18 (45 kDa) are members of the type I acidic subfamily expressed in cornified epithelia, non-cornified stratified squamous epithelia and simple epithelial cells respectively. Cytokeratin peptide 19 (40 kDa) is a type I keratin which can be expressed in both simple and in broad cells of stratifying epithelia at specific sites. |
| Immunogen: | mixture of several monoclonal cytokeratin clones. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |