CelLytic™ P Cell Lysis Reagent
SIGMA/C2360
Synonym: Plant cell lysis reagent
Product Type: Chemical
Figure 1. Compatibility with Gel Shift Assay.
Protein extracts were prepared with the CelLytic P reagent from spinach leaves. A double stranded 32P-labeled CREB oligonucleotide probe was incubated with 28 μg of the whole cell extract (lanes 2-5) or without whole cell extract (lane 1, free probe). Binding reactions with the extracts were performed in the absence [–] of competitor oligonucleotide (lanes 1-2) or in the presence of 100- or 500-fold excess of unlabeled CREB binding motif oligonucleotide (specific competitor [SP], lane 3 and lane 4, respectively) or in the presence of 100-fold excess of unlabeled oligonucleotide (non specific competitor, [NS] lane 5). Binding reactions were run on a non-denaturing 6% polyacrylamide gel, dried and imaged on X-ray film. The arrows indicate the CREB-DNA complex and the free probe.
Protein extracts were prepared with the CelLytic P reagent from spinach leaves. A double stranded 32P-labeled CREB oligonucleotide probe was incubated with 28 μg of the whole cell extract (lanes 2-5) or without whole cell extract (lane 1, free probe). Binding reactions with the extracts were performed in the absence [–] of competitor oligonucleotide (lanes 1-2) or in the presence of 100- or 500-fold excess of unlabeled CREB binding motif oligonucleotide (specific competitor [SP], lane 3 and lane 4, respectively) or in the presence of 100-fold excess of unlabeled oligonucleotide (non specific competitor, [NS] lane 5). Binding reactions were run on a non-denaturing 6% polyacrylamide gel, dried and imaged on X-ray film. The arrows indicate the CREB-DNA complex and the free probe.
| description | For plant cell lysis |
| form | solution |
| Quality Level | 200  |
| storage temp. | room temp |
| Application: | CelLytic™ P Cell Lysis Reagent has been used for lysate preparation for cell lysis. |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | CelLytic™ P is a non-ionic detergent-based reagent, which offers a convenient method for efficient plant cell lysis and protein solubilization. It is a non-denaturing reagent and maintains protein immunoreactivity and biological activity. CelLytic™ P is efficient, rapid, and ready to use. It contains bicine buffer, which is preferable for many biological activities. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 - H412 |
| Precautionary statements | P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 41116134 |