Monoclonal Anti-Carcinoembryonic Antigen antibody produced in mouse
SIGMA/C2331 - clone C6G9, ascites fluid
MDL Number: MFCD00162210
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human colon adenocarcinoma sections. Fixed sections were stained with 1:8,000 Monoclonal Anti-human Carcinoembryonic Antigen, Clone: C6G9 (Cat. No. C2331) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting CEA preparation extract was separated on SDS-PAGE and probed with Monoclonal AB To CEA (MO)CL:C6G9 (PREPK) (Cat. No. C2331). The antibody was developed with Alk Phos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/1500
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C6G9, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:8,000 using human colon carcinoma tissue | |
| western blot: suitable | |
| UniProt accession no. | P13688  |
| Application: | Monoclonal Anti-Carcinoembryonic Antigen antibody is suitable for use in immunohistochemistry (1:8,000 using formalin-fixed, paraffin-embedded sections of human colon carcinoma tissue). |
| Application: | Monoclonal Anti-Carcinoembryonic Antigen antibody produced in mouse has been used in western blot analysis and surface plasmon resonance (SPR) based immunoassay. |
| Biochem/physiol Actions: | Carcinoembryonic Antigen (CEA) inhibits tumor growth in colon, hepatoma and prostate cancers. |
| Biochem/physiol Actions: | The antibody reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissue including colorectal, lung and breast tumors. It also stains medullary carcinomas of the thyroid. The product reacts strongly with the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. A weak reactivity is seen with normal colon mucosa and occasionally with bile canalicular and pancreatic acinar cells, but not with other tested tissue including term placenta. There is no cross-reactivity with non-specific cross-reacting antigen (NCA) present in granulocytes, using immunoblotting, flow cytometry and immunohistochemical techniques. The antibody recognizes an epitope that is resistant to 30 minute oxidation by 1% sodium periodate solution in routine formalin-fixed and paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes improves immunohistochemical staining with the antibody. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Carcinoembryonic Antigen (CEA) is a member of the immunoglobulin superfamily that regulates cellular signaling and adhesion. CEA inhibits cell differentiation and subsequently facilitates tumor growth . Thus, CEA has been implicated in various cancers, including colorectal, lung and breast tumors Monoclonal Anti-Human Carcinoembryonic Antigen (CEA) reacts specifically with human CEA (CD66e, 180 kDa) from different types of malignant tissues. The antibody detects an epitope which is resistant to 30 minute oxidation by 1% sodium periodate solution in formalin-fixed, paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes enhances immunohistochemical staining with the antibody.The product reacts with medullary thyroid cancers and also associates with cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. Additionally, the product reacts weakly with normal colon mucosa and occasionally reacts with bile canalicular and pancreatic acinar cells. However, the antibody does not react with other tested tissue such as term placenta, nor does it bind to non-specific cross-reacting antigen (NCA) in granulocytes. |
| General description: | Carcinoembryonic Antigen (CEA) is located on human chromosome 19q13.2. |
| Immunogen: | carcinoembryonic antigen (CEA) isolated from a human colon adenocarcinoma cell line. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |