Concanavalin A from Canavalia ensiformis (Jack bean)

SIGMA/C2272 - biotin conjugate, Type IV, lyophilized powder

Synonym: ConA

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-C2272-2MG	2 mg	$54.10 1/EA		Add To Favorites
45-C2272-10MG	10 mg	$176.00 1/EA		Add To Favorites

This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

Properties
Descriptions
Safety Info.
Basic Data

biological source	Canavalia ensiformis
composition	Protein, ~85% ε1%/280
conjugate	biotin conjugate
extent of labeling	4-8 mol biotin per mol protein
form	lyophilized powder
impurities	Carbohydrate, essentially free
Quality Level	200
storage temp.	2-8°C
type	Type IV

Analysis Note:	Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Application:	Concanavalin A from Canavalia ensiformis (Jack bean) has been used: • in the colabelling of fischer rat thyroid (FRT) epithelia(104) • to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice(105) • to detect glycoproteins containing α-D-mannose, α-D-glucose when used with avidin or streptavidin detection system(106)
Biochem/physiol Actions:	Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Biochem/physiol Actions:	Concanavalin A (Con A) bound to glycoenzyme has wide applications and can be dissociated in the presence of high sugar levels. Con A induces hepatic inflammation. It stimulates macrophages and promotes cytokine production. Con A also exhibits anti-hepatoma effect and could be used effectively as a vaccine for treating cancer.
General description:	Concanavalin A is isolated from the from Canavalia ensiformis (Jack bean). It is a mitogenic lectin with a dome-like structures and is a tetrameric protein.
Packaging:	Package size based on protein content
Physical form:	Contains sodium citrate

Symbol	GHS07
Signal word	Warning
Hazard statements	H317
Precautionary statements	P280
Hazard Codes	Xn
Risk Statements	42/43
Safety Statements	22-36/37-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352202

Choose a favorite list for this item:

Catalog Number	Description	Price

		$

Please choose a list :

Continue Shopping

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.