Collagenase from Clostridium histolyticum
SIGMA/C2139 - suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Synonym: Clostridiopeptidase A
CAS Number: 9001-12-1
EC Number: 232-582-9
MDL Number: MFCD00130830
Product Type: Chemical
Sigma collagenases are fully characterized by enzymatic activity. Many are specifically tested for suitability for cell dissociation and cell viability for hepatocytes, adipocytes, and pancreatic cells.
Unit Definition One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions.
| application(s) | diagnostic assay manufacturing |
| biological source | Clostridium histolyticum |
| form | powder |
| mol wt | 68-125 kDa |
| Quality Level | 200  |
| specific activity | ≥125 CDU/mg solid |
| 0.5-5.0 FALGPA units/mg solid | |
| storage temp. | −20°C |
| suitability | suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV) |
| type | Type VIII |
| Analysis Note: | Also contains clostripain, nonspecific neutral protease and tryptic activities. |
| Application: | Collagenase may be used: • for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE) • for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. • in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. • for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue |
| Biochem/physiol Actions: | Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethy |
| Biochem/physiol Actions: | Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue. Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X represents a neutral amino acid. |
| General description: | Clostridium histolyticum is a pathogenic clostridium that produces crude collagenases. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain. |
| Other Notes: | One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 100, 500 mg in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥125 CDU/mg solid; specific activity: 0.5-5.0 FALGPA units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |