DMT-2′O-Methyl-rC(ac) Phosphoramidite
SIGMA/C213100
Synonym: N-acetyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-cytidine, 3′-[2-cyanoethyl N,N-
CAS Number: 199593-09-4
Empirical Formula (Hill Notation): C42H52N5O9P
Molecular Weight: 801.86
MDL Number: MFCD12912403
Linear Formula: C42H52N5O9P
Product Type: Chemical
| λ | conforms (UV/VIS Identity) |
| assay | ≥99% (31P-NMR) |
| ≥99.0% (reversed phase HPLC) | |
| biological source | non-animal source (no BSE/TSE risk) |
| color | white to off-white |
| form | powder |
| impurities | ≤0.1% single unspecified Impurity (reversed phase HPLC) |
| ≤0.3% mC2 (reversed phase HPLC, Hydrolysate) | |
| ≤0.3% mC3 (reversed phase HPLC, DMT-rC(ac)me) | |
| ≤0.3% mC4 (reversed phase HPLC, DMT-rC(ac)me-Ac) | |
| ≤0.3 wt. % water content (Karl Fischer) | |
| ≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
| ≤1.0% mC1 (reversed phase HPLC, DMT-rC(ac)me-DMT) | |
| ≤3 wt. % residual Solvent content | |
| InChI | 1S/C42H52N5O9P/c1-28(2)47 |
| InChI key | WNWUMIPFLOKTEZ-IXZONTHDSA |
| nucleoside profile | base: cytidine base protecting group: acetyl 2' protecting group: methyl 5' protecting group: DMT deprotection: fast/standard |
| product line | Proligo Reagents |
| Quality Level | 300  |
| SMILES string | P(N(C(C)C)C(C)C)(O[C@@H]1 |
| storage temp. | -10 to -25°C |
| suitability | conforms to structure for H-NMR |
| conforms to structure for LC-MS | |
| technique(s) | oligo synthesis: suitable |
| Application: | DMT-2′O-Methyl-rC(ac) Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance. |
| General description: | DMT-2′O-Methyl-rC(ac) Phosphoramidite belongs to the group of 2′O-Methyl RNA Phosphoramidites. 2′O-Methyl RNA monomers are compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. 2′O-Methyl RNA is a nucleic acid analog that is characterized by the exceptional hybridization properties that it imparts with complementary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids. 2′O-Methyl RNA monomers feature methoxy groups at the 2′-position. The methoxy groups are perfectly stable in all conditions employed in the assembly of oligonucleotides by automated phosphoramidite synthesis, and in all standard alkaline deprotection conditions. |
| Other Notes: | It has widespread applications in the fields of: • Diagnostic probes • Aptamer and ribozyme development • Mixed 2′O-Methyl-RNA/DNA antisense molecules |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
| Storage Temp. | -10 to -25°C |
| UNSPSC | 41116105 |