CMP-Sialic Acid Synthetase from Neisseria meningitidis group B
SIGMA/C1999 - recombinant, expressed in E. coli BL21, ≥10 units/mg protein
Synonym: CTP: N-Acylneuraminate cytidylyltransferase
MDL Number: MFCD01323074
Product Type: Chemical
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| form | lyophilized solid |
| mol wt | 26.0 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli BL21 |
| shipped in | dry ice |
| specific activity | ≥10 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm). |
| Application: | The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives. |
| Biochem/physiol Actions: | Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg2+ is essential for the catalytic functionality of CMP-sialic acid synthetase. |
| General description: | Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa. |
| Other Notes: | One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0. |
| Physical form: | Supplied as a lyophilized powder containing Tris-HCl and NaCl. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.43 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |