Anti-Coilin antibody, Mouse monoclonal
SIGMA/C1862 - ~1.5 mg/mL, clone pδ, purified from hybridoma cell culture
MDL Number: MFCD03792415
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: P68 RNA helicase (DDX5) alters activity of cis- and trans-acting factors of the alternative splicing of H-Ras. By: Camats, M., Guil, S., et al. in PLoS One, 2008. PubMed ID: 18698352 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0002926), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Monoclonal Anti-Coilin Clone: pδ (Cat. No. C1862). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting HeLa cells nuclear extract were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-Coilin antibody produced in Mouse, Clone: p-δ (Cat. No. C1862). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1682).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | pδ, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~80 kDa by SDS-PAGE |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| microarray: suitable | |
| western blot: 1-2 μg/mL using HeLa nuclear extract | |
| UniProt accession no. | P38432  |
| Application: | Monoclonal Anti-Coilin antibody produced in mouse has been used in: • immunoblotting • immunocytochemistry • cell microinjection • fluorescence imaging • indirect immunofluorescence |
| Biochem/physiol Actions: | Mutating Serine-202 to Aspartate causes the disappearance of coiled bodies and a redistribution of coilin to intranucleolar domains. Coilin plays a key role in ribonucleoprotein and Cajal body formation. |
| Biochem/physiol Actions: | The antibody recognizes the C-terminal region of human coilin and does not recognize mouse coilin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Coilin (mouse IgG1 isotype) is derived from the pδ hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with coilin. Coilin contains two nuclear localization sequences (NLS) (at amino acid 107-112 and 181-198) and several serine residues that are phosphorylated in vivo. The description of specific intranuclear structures known today as Cajal bodies was first published in 1903 by the neuro-cytologist Ramon-γ-Cajal who discovered that neurons contained spherical structures of around 0.5 μm in diameter that were often associated with nucleoli, nucleolar accessory bodies. It was found that patients with auto-antibodies against coiled bodies recognize a protein of 80 kDa termed p80-coilin. Nuclear antigens shown to colocalize with p80 coilin in Cajal bodies include basal transcription factors, cell cycle factors (cdks), splicing snRNPs and nucleolar factors including snoRNP. |
| Immunogen: | C-terminal (389 amino acids) human coilin |
| Physical form: | Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |