Anti-CD4 antibody, Mouse monoclonal
SIGMA/C1805 - clone Q4120, purified from hybridoma cell culture
Synonym: Anti-CD4 antibody, Mouse monoclonal
Product Type: Chemical
Flow Cytometry 1×106 Human lymphocytes were labeled with 5 μg/test Anti-CD4 antibody, Mouse monoclonal, Clone: Q4120 (Cat. No. C1805) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
Flow Cytometry 1×106 Human monocytes were labeled with 5 μg/test Anti-CD4 antibody, Mouse monoclonal, Clone: Q4120 (Cat. No. C1805) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
Flow Cytometry 1×106 Human granulocytes were labeled with 5 μg/test Anti-CD4 antibody, Mouse monoclonal, Clone: Q4120 (Cat. No. C1805) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Q4120, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 59 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5 μL using 1 × 106 cells |
| UniProt accession no. | P01730  |
| Application: | Monoclonal Anti-CD4 antibody produced in mouse has been used in indirect immunofluorescent staining. |
| Biochem/physiol Actions: | Cluster of differentiation 4 (CD4) molecule binds to the major histocompatibility complex (MHC) class II molecules during the interaction of CD4+ T cells with antigen presenting cells or with target cells. It also serves as a high affinity cellular receptor for the GP 120 envelope glycoprotein of the human immunodeficiency virus (HIV-1, HIV-2). The CD4 molecule is involved in the adhesion of T-lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins. Lower levels of CD4 have been detected in monocytes, macrophages and granulocytes. |
| Biochem/physiol Actions: | Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase. |
| General description: | Monoclonal Anti-Human CD4 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with CD4-Transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. Cluster of differentiation 4 (CD4) human cell surface glycoprotein (59kDa) belongs to the immunoglobulin superfamily. It is expressed on the helper/inducer T cell subset, which is found on the majority of peripheral blood neutrophils, most cortical and mature medullary thymocytes, microglial cells, dendritic cells and on some malignancies of T cell origin. |
| Immunogen: | CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |