Anti-Calcium Channel (α1D Subunit) (L-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit
SIGMA/C1728 - affinity isolated antibody, lyophilized powder
Synonym: Anti-CACH3; Anti-CACN4; Anti-CACNL1A2; Anti-CCHL1A2; Anti-Cav1.3; Anti-PASNA; Anti-SANDD
MDL Number: MFCD02095953
Product Type: Chemical
Immunohistochemistry Anti-Cav1.3: Cat. No. C1728: Immunohistochemical staining of adult Rat dorsal root ganglion (DRG) with Anti-Cav1.3 antibody. Staining appears in clusters of cells (green) but not in axons. Staining of neurofilament 200 demonstrates partial overlap with Cav1.3 in neuronal staining but not in axons (red).
Immunoblotting Anti-Cav1.3: Cat. No. C1728: Western blot analysis of Rat brain membranes. Lanes 1. Anti-Cav1.3 antibody, (1:200). 2. Anti-Cav1.3 antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 100  |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1:30-1:150 |
| immunoprecipitation (IP): suitable | |
| western blot (chemiluminescent): 1:100-1:200 | |
| UniProt accession no. | Q99246  |
| Application: | Anti-Calcium Channel (α1D Subunit) (L-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit is suitable for the following applications: • immunocytochemistry at a dilution of 1:30-1:150 • immunoprecipitation • western blot (chemiluminescent) at a dilution of 1:100-1:200 |
| Biochem/physiol Actions: | Cav1.3 also known as the calcium channel, voltage-dependent, L type, α1D subunit (CACNA1D), is a human gene. It responds more sensitively to depolarization than those of Cav1.2 or Cav3.1. Cav1.3 is required for proper hearing as well as sinoatrial node and brain function. It undergoes extensive alternative splicing. Alternative splicing in the C terminus and drastically modifies gating properties of the channel. The L-type calcium channel Cav1.3 is important in human glucose-induced insulin secretion and common variants in CACNA1D may contribute to type 2 diabetes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide corresponding to amino acids 859-875 of rat α1D (Accession P27732). This epitope is highly conserved in human and hamster. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |