Anti-C-Reactive Protein antibody, Mouse monoclonal
SIGMA/C1688 - clone CRP-8, purified from hybridoma cell culture
Synonym: Monoclonal Anti-C-Reactive Protein antibody produced in mouse; Anti-CRP
MDL Number: MFCD00162183
Product Type: Chemical
Immunoblotting Humna CRP positive serum was separated on SDS-PAGE and probed with Monoclonal AB to C-Reactive Protein (MO) CL:CRP-8 (Cat. No. C1688). The antibody was developed with Biotin APA M Fab ads Hu/Rat conjugate (Cat. No. B0529), Alkaline Phosphatase ExtrAvidin (Cat. No. E2636) and a NBT/BCIP substrate. Antibody dilution 1/400
ELISA Monoclonal Anti-C-Reactive Protein antibody produced in Mouse, Clone: CRP-8 (Cat. No. C1688), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect C-Reactive Protein by indirect ELISA. Reactivity is observed with CRP (C-Reactive Protein), but not with SAP (Serum Amyloid P component).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CRP-8, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 24 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: 1:40,000-80,000 | |
| western blot: 1:200-400 | |
| UniProt accession no. | P02741  |
| Application: | • It has been used in immunoblotting assay, to identify monomeric CRP. • In time-resolved fluorometric assay. |
| Application: | Monoclonal Anti-C-Reactive Protein antibody produced in mouse is suitable for the following: • dot blot analysis • indirect ELISA at a working dilution of 1:40,000 • western blotting at a working dilution of 1:200 |
| Biochem/physiol Actions: | C-reactive protein (CRP) shows essential scavenging and metabolic activity and is also associated with defence mechanism. It activates the complement pathway by binding to calcium-dependent exogenous and autologous molecules containing phosphocholine (PC). It may have pathogenic effects and is a practical prognostic marker in patients with hepatocellular carcinoma (HCC). The CRP diagnostic value is higher for acute pyelonephritis than lower urinary tract infection. Thus, it helps in differentiating between upper and lower urinary tract infection. It is considered best for predicting the risk of cardiovascular disease.. |
| Biochem/physiol Actions: | The antibody recognizes an epitope located on the 24 kDa subunit of denatured and reduced CRP. It does not cross-react with human serum amyloid P component (SAP), human haptoglobin, human α1-acid glycoprotein, human IgG, and CRP from Limulus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | C-reactive protein (CRP) is a member of the pentraxin family of proteins and synthesized by the liver. It is also known as PTX1. The CRP gene is mapped to human chromosome 1q23.2. |
| Immunogen: | human C-reactive protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |