Cytochrome b5 human
SIGMA/C1427 - recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)
Synonym: Cytochrome b5
MDL Number: MFCD02687283
Product Type: Chemical
Cytochrome P450 protein complex: CYP450, cytochrome b5 and NADPH reductase
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | solution |
| mol wt | 16 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli (histidine-tagged) |
| shipped in | dry ice |
| storage temp. | −70°C |
| suitability | suitable for molecular biology |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | P00167  |
| Analysis Note: | Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b5. |
| Biochem/physiol Actions: | Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen. |
| Biochem/physiol Actions: | Cytochrome b5 is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown. |
| Biochem/physiol Actions: | Membrane-bound heme-containing protein; enhances catalytic efficiency of some P450 isoforms. |
| General description: | Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary. |
| Physical form: | Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12161501 |